Bonus
Answer the following about the analysis of tetrodotoxin, for 10 points each.
[10h] Woodward determined tetrodotoxin’s structure by oxidizing it into the picrate salt of this ion. The hydrochloride or thiocyanate of this ion can show up as an undesirable 230-nanometer peak following DNA extractions.
ANSWER: guanidine [or guanidinium; accept guanidinium hydrochloride or guanidinium thiocyanate or guanidinium peak]
[10e] Tetrodotoxin’s toxicity comes from its ability to block voltage-gated channels of this metal. ATP drives the active transport of three ions of this metal with two ions of potassium across a namesake pump.
ANSWER: sodium [accept Na; accept sodium-potassium pump]
[10m] Tetrodotoxin’s mechanism can be determined by using a “voltage clamp” of this molecule to inhibit sodium and potassium channels. Either cesium chloride or this molecule is used to form a concentration gradient in isopycnic centrifugation experiments.
ANSWER: sucrose [accept sucrose-gap voltage clamping; accept sucrose gradient centrifugation]
<Biology>
Answerlines and category may not exactly match the version played at all sites
Conversion
| Team | Opponent | Part 1 | Part 2 | Part 3 | Total | Parts |
|---|---|---|---|---|---|---|
| Arizona State | North Carolina B | 0 | 10 | 10 | 20 | EM |
| Columbia A | Winona State | 0 | 10 | 0 | 10 | E |
| Florida | Yale | 0 | 10 | 0 | 10 | E |
| Georgia Tech | Waterloo B | 0 | 10 | 0 | 10 | E |
| Illinois A | Chicago B | 0 | 10 | 10 | 20 | EM |
| Johns Hopkins | Virginia Tech | 0 | 10 | 10 | 20 | EM |
| LSE | North Carolina A | 0 | 10 | 0 | 10 | E |
| MIT | Cornell B | 0 | 0 | 0 | 0 | |
| Maryland | Cornell A | 0 | 10 | 0 | 10 | E |
| Michigan | British Columbia | 0 | 10 | 0 | 10 | E |
| NYU | Toronto C | 0 | 10 | 0 | 10 | E |
| Northwestern | UCF | 10 | 10 | 10 | 30 | HEM |
| Ohio State | UC Berkeley B | 0 | 10 | 0 | 10 | E |
| Rutgers | Virginia | 0 | 10 | 10 | 20 | EM |
| Stanford | Georgia State | 0 | 10 | 10 | 20 | EM |
| Toronto A | Texas | 0 | 10 | 10 | 20 | EM |
| Toronto B | Chicago A | 0 | 10 | 0 | 10 | E |
| UC Berkeley A | Columbia B | 0 | 10 | 10 | 20 | EM |
| Vanderbilt | Harvard | 0 | 0 | 0 | 0 | |
| WUSTL A | Illinois B | 0 | 10 | 0 | 10 | E |
| Waterloo A | Ottawa | 0 | 10 | 0 | 10 | E |
Summary
| Tournament | Exact Match? | Heard | PPB | Easy % | Medium % | Hard % |
|---|---|---|---|---|---|---|
| 2025 ACF Nationals | Yes | 21 | 13.33 | 91% | 38% | 5% |