Bonus

Answer the following about the analysis of tetrodotoxin, for 10 points each.
[10h] Woodward determined tetrodotoxin’s structure by oxidizing it into the picrate salt of this ion. The hydrochloride or thiocyanate of this ion can show up as an undesirable 230-nanometer peak following DNA extractions.
ANSWER: guanidine [or guanidinium; accept guanidinium hydrochloride or guanidinium thiocyanate or guanidinium peak]
[10e] Tetrodotoxin’s toxicity comes from its ability to block voltage-gated channels of this metal. ATP drives the active transport of three ions of this metal with two ions of potassium across a namesake pump.
ANSWER: sodium [accept Na; accept sodium-potassium pump]
[10m] Tetrodotoxin’s mechanism can be determined by using a “voltage clamp” of this molecule to inhibit sodium and potassium channels. Either cesium chloride or this molecule is used to form a concentration gradient in isopycnic centrifugation experiments.
ANSWER: sucrose [accept sucrose-gap voltage clamping; accept sucrose gradient centrifugation]
<Biology>
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Summary

TournamentExact Match?HeardPPBEasy %Medium %Hard %
2025 ACF NationalsYes2113.3391%38%5%