Bonus
These constructs are most commonly added to cells via either heat shock or electroporation protocols. For 10 points each:
[10e] Name these circular pieces of DNA that can be transformed into bacteria to express new genes in the lab.
ANSWER: plasmids
[10m] Researchers balance expression and stability by choosing plasmid origins associated with a high, medium, or low value for this quantity. This quantity measures the number of individual instances of a plasmid in a cell.
ANSWER: plasmid copy number
[10h] Plasmids with a particularly low copy number may require this process to remain stable in populations. ParA and addiction modules like the hok/sok system are common elements that ensure this process occurs successfully.
ANSWER: plasmid segregation [accept plasmid partition or plasmid partition systems; prompt on replication or self-replicating]
<Biology>
Answerlines and category may not exactly match the version played at all sites
Conversion
Team | Opponent | Part 1 | Part 2 | Part 3 | Total | Parts |
---|---|---|---|---|---|---|
Columbia A | Stanford | 10 | 10 | 0 | 20 | EM |
Cornell A | Iowa State | 10 | 10 | 0 | 20 | EM |
Cornell B | Maryland | 10 | 0 | 0 | 10 | E |
Florida | Arizona State | 10 | 10 | 0 | 20 | EM |
Georgia Tech | MIT | 10 | 10 | 0 | 20 | EM |
Harvard | Minnesota | 10 | 0 | 0 | 10 | E |
Illinois B | North Carolina B | 10 | 10 | 0 | 20 | EM |
Johns Hopkins | Toronto B | 10 | 10 | 0 | 20 | EM |
LSE | WUSTL B | 10 | 0 | 0 | 10 | E |
Michigan | Vanderbilt | 10 | 10 | 0 | 20 | EM |
NYU | Georgia State | 10 | 0 | 0 | 10 | E |
North Carolina A | Chicago A | 10 | 10 | 0 | 20 | EM |
Northwestern | Illinois A | 10 | 10 | 0 | 20 | EM |
Ohio State | Winona State | 10 | 0 | 0 | 10 | E |
Penn State | Waterloo B | 10 | 0 | 0 | 10 | E |
Rutgers | UC Berkeley A | 10 | 10 | 0 | 20 | EM |
Texas | Chicago B | 10 | 0 | 0 | 10 | E |
Toronto A | Indiana | 10 | 10 | 0 | 20 | EM |
Toronto C | Virginia Tech | 10 | 10 | 0 | 20 | EM |
UC Berkeley B | British Columbia | 10 | 10 | 0 | 20 | EM |
UCF | RIT | 10 | 0 | 0 | 10 | E |
Virginia | Columbia B | 10 | 0 | 0 | 10 | E |
WUSTL A | Waterloo A | 10 | 10 | 0 | 20 | EM |
Yale | Ottawa | 10 | 0 | 0 | 10 | E |
Summary
Tournament | Exact Match? | Heard | PPB | Easy % | Medium % | Hard % |
---|---|---|---|---|---|---|
2025 ACF Nationals | Yes | 24 | 15.83 | 100% | 58% | 0% |