Bonus
This technique relies on an interaction that occurs when a donor chromophore in an excited state transfers energy to a nearby acceptor chromophore. For 10 points each:
[10m] Name this technique that is used to determine the presence of protein–protein interactions since its efficiency is inversely proportional to the sixth power of distance.
ANSWER: FRET (“fret”) [or forster resonance energy transfer; accept, but DO NOT REVEAL, fluorescence resonance energy transfer]
[10e] The chromophores in FRET exhibit this phenomenon in which a molecule absorbs light and then emits it at a longer wavelength. It is contrasted with phosphorescence.
ANSWER: fluorescence [accept word forms like fluorescent]
[10h] Most instances of FRET that don’t use Cy3 and 5 use a cleavage assay that makes use of tandem dimer of these two chromophores linked by a protease. One of these two chromophores emits light at 525 nanometers and the other emits light at 414 nanometers.
ANSWER: CFP AND YFP [accept cyan fluorescent protein and yellow fluorescent protein in place of “CFP” or “YFP”, respectively]
<Biology>
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Conversion
| Team | Opponent | Part 1 | Part 2 | Part 3 | Total | Parts |
|---|---|---|---|---|---|---|
| North Carolina A | Cornell A | 10 | 10 | 0 | 20 | ME |
Summary
| Tournament | Exact Match? | Heard | PPB | Easy % | Medium % | Hard % |
|---|---|---|---|---|---|---|
| 2025 ACF Nationals | Yes | 1 | 20.00 | 100% | 100% | 0% |